
Free Hemoglobin (FHb) Assay Kit (Colorimetric Method)
PN5720
Perfumer
50T/48S
China
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Plasma free hemoglobin (FHb) refers to the amount of hemoglobin measured in plasma. Hemoglobin is typically contained within red blood cells; it is released when these cells are destroyed. Measuring FHb levels helps assess the extent of red blood cell destruction in patients with hemolytic anemia. Elevated plasma free hemoglobin is observed in intravascular hemolysis, thalassemia, and autoimmune hemolytic anemia.
Product benefits:
1 Fast and easy: Takes about 50 minutes.
2 Sample Volume: This method uses a sample volume of 150 μL.
3 High stability: The kit remains valid for 3 months when stored at 2~8℃.
4, High reproducibility: Coefficient of Variation (CV) = 1.7%.
5 Recovery Test: X = 102%.
6: Less affected by external factors: minimal interference and high repeatability.
7 Broad testing scope: can test animal serum/plasma.
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For free hemoglobin in plasma samples, as well as in lysates or homogenates of cells and tissues. Based on the o-tolidine colorimetric method: ferrous heme in hemoglobin exhibits peroxidase-like activity, catalyzing the oxidation of o-tolidine to a blue-green product with an absorption peak at 63 nm; under acidic conditions, the product turns yellow with an absorption peak at 435nm,. The intensity of the yellow color is proportional to the hemoglobin concentration.
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