Free Hemoglobin (FHb) Detection Kit (Orthotolidine Colorimetric Method)

For free hemoglobin in plasma samples, as well as in lysates or homogenates of cells and tissues. Based on the o-tolidine colorimetric method: ferrous heme in hemoglobin exhibits peroxidase-like activity, catalyzing the oxidation of o-tolidine to a blue-green product with an absorption peak at 63 nm; under acidic conditions, the product turns yellow with an absorption peak at 435nm,. The intensity of the yellow color is proportional to the hemoglobin concentration.

¥227

Item Number

PM23602

Brand

Perfumer

Specifications & Packaging

50TB

Origin

China

Inventory Status

In Stock

Status

On Sale

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Hemoglobin (Hb or HGB) is a protein in higher organisms responsible for transporting oxygen and giving blood its red color. It consists of four chains: two alpha chains and two beta chains. Each chain contains a heme group with an iron atom. Hb readily binds to oxygen in high-oxygen environments and releases it in low-oxygen environments. This property enables red blood cells to transport oxygen. During intravascular hemolysis, the concentration of free hemoglobin in plasma increases.

The Free Hemoglobin Colorimetric Assay Kit (O-tolidine Method) operates on the principle that ferrous heme in hemoglobin exhibits peroxidase-like activity. In the presence of an oxidizing agent, it catalyzes O-tolidine to form a cyan or blue product with an absorption peak at 630 nm. Under acidic conditions, this product turns yellow with an absorption peak at 435 nm; deeper yellow coloration indicates higher free hemoglobin (FHB) levels, while lighter coloration indicates lower levels. By measuring absorbance at 435 nm using spectrophotometry, plasma FHB concentration can be calculated. This kit is primarily designed for plasma samples but can also be used to determine FHB content in cell or tissue lysates and homogenates.

This kit is for research use only and is not intended for clinical diagnosis or other applications.