Hematoxylin and Eosin (H&E) Staining Kit

Hematoxylin is a natural dye extracted from logwood and widely used in various formulations—such as Mayer's, Gill's, and Harris's—to stain cell nuclei, mitochondria, mucins, elastic fibers, muscle, collagen, myelin sheaths, and phospholipids. Its staining mechanism relies on the binding of positively charged basic hematoxylin to negatively charged acidic substances, resulting in a blue coloration.

Eosin (also known as erythrosine) is a red fluorescent dye that dissociates in water into negatively charged anions. These anions bind to positively charged cations in protein amino groups, staining cell cytoplasm, red blood cells, collagen, muscle fibers, connective tissue, and eosinophilic granules various shades of red or pink. As an acidic dye, it is commonly used in Hematoxylin and Eosin (H&E) staining as a counterstain for hematoxylin.

Hematoxylin and Eosin (H&E) staining colors the cytoplasm red, nuclei blue-purple, and red blood cells orange-red, while other components appear in varying shades of red. It is widely used for staining tissue sections, blood smears, bone marrow sections, and cell smears in immunohistochemistry. As one of the most routine staining methods, H&E is essential in cell biology, histology, and pathology.